DotMatch Next 10 Industry Exposure Wins

This playbook builds on the first five exposure wins in docs/industry-exposure.md. It turns the next adoption layer into concrete assets that can be used by maintainers, workflow reviewers, core facilities, CRISPR teams, and assay developers without expanding DotMatch’s public claim boundary.

Use docs/industry-exposure-plan.json as the machine-readable checklist for these ten wins. Keep private conversations, unmerged PRs, and internal examples out of public adopter evidence until the rules in docs/adopters/README.md are satisfied.

1. Evaluator Decision Tree

Use this when someone asks whether DotMatch is relevant.

Question

If yes

If no

Do you already know the expected short target sequences?

Continue.

DotMatch is probably not the right first tool.

Is the read window fixed, scaffolded, or inferable?

Use count, demux, assay, barcode, or panel docs.

Use extraction or preprocessing before DotMatch.

Do ambiguous, unmatched, or invalid reads affect interpretation?

Lead with reliability and output artifacts.

Use DotMatch only if ordinary count outputs still help.

Do you need genome coordinates, CIGAR strings, UMI deduplication, or hit statistics?

Use established downstream tools.

DotMatch can own the assignment layer.

Do you need a public pipeline integration?

Start with workflow submissions.

Start with local install and methods text.

2. Persona One-Pagers

Core Facilities

  • Promise: visible assignment QC for known guides, barcodes, and panels.

  • Proof path: homepage, barcode troubleshooting, evidence gallery, packaging.

  • First command: dotmatch barcode autopsy ...

  • Review artifact: HTML report plus TSV/JSON outputs for lab handoff.

CRISPR Screen Teams

  • Promise: guide counts with explicit ambiguity and unmatched diagnostics.

  • Proof path: CRISPR tutorial, public CRISPR evidence, methods citation text.

  • First command: dotmatch crispr-count ...

  • Review artifact: count matrix, sample QC, top unmatched, MAGeCK-compatible table.

Workflow Maintainers

  • Promise: stable TSV, JSON, FASTQ, and HTML artifacts for wrappers.

  • Proof path: workflow submission pack, schemas, MultiQC parser, release gates.

  • First command: make workflow-examples-ready

  • Review artifact: wrapper fixture, command log, expected output contract.

Assay Developers

  • Promise: barcode panel design and correction-safety review before sequencing.

  • Proof path: barcode panel design docs, assay evidence, panel report.

  • First command: dotmatch panel design ...

  • Review artifact: design report, collision tables, plate layout, lab README.

3. Integration Target Tracker

Track external integration work without calling it adoption too early.

Target

Why it matters

Ready asset

Public state to record

nf-core modules

High-trust workflow reuse and container automation

docs/workflow-submissions.md

Merged PR or released module page

MultiQC module

Makes DotMatch visible in existing pipeline reports

python/dotmatch/multiqc.py

Released plugin or upstream integration

Galaxy/IUC

Reaches core facilities and wet-lab users

Galaxy wrapper examples

IUC acceptance or ToolShed publication

Snakemake wrapper

Easy lab workflow reuse

Snakemake example workflow

Public wrapper or external lab pipeline

bio.tools entry

Searchable bioinformatics registry presence

Homepage and metadata

Accepted bio.tools record

4. Reviewer Evidence Packet

Send this packet when a maintainer, reviewer, procurement evaluator, or PI asks what is real today.

  • Positioning: homepage.

  • Install proof: PyPI, Bioconda, packaging notes.

  • Output contract: schemas and command reference.

  • Claim boundary: scientific claims and trust/scope docs.

  • Evidence: evidence gallery and benchmark pages.

  • Citation: methods and citation template.

  • Adoption rules: adopter notes and workflow adoption JSON.

5. Conference Abstracts

Short Abstract

DotMatch is a deterministic known-target sequencing assignment toolkit for fixed read windows such as CRISPR guides, inline barcodes, feature tags, primers, and panel targets. It reports unique, ambiguous, unmatched, and invalid read outcomes so assignment failures remain visible in workflow artifacts.

Methods Abstract

Known-target sequencing workflows often collapse assignment decisions into count tables, making ambiguous reads, shifted windows, unsafe correction, and recurring unmatched sequences hard to inspect. DotMatch separates the assignment layer from downstream interpretation: it compares configured read windows with known short DNA targets, records explicit read outcomes, and writes TSV, JSON, FASTQ, and HTML artifacts for workflow review. Public claims are scoped to checked repository evidence and release gates.

Core Facility Abstract

DotMatch helps sequencing cores review known-target assays before results leave the facility. It supports guide counting, inline barcode demultiplexing, barcode panel design, and assignment autopsy reports while preserving ambiguity, unmatched reads, and invalid extraction windows as visible QC signals.

6. Social And Forum Pack

Technical Thread

DotMatch focuses on one layer: assigning fixed read windows to known short DNA
targets.

Why that matters:
1. unique reads can be counted
2. ambiguous reads stay out of forced calls
3. unmatched reads remain reviewable
4. invalid windows are visible QC failures

Docs: https://dotmatch.readthedocs.io/
Homepage: https://dnncha.github.io/dotmatch

Forum Prompt

I am looking for feedback from teams that run known-target sequencing assays:
CRISPR guide counting, inline barcode demultiplexing, feature tags, primers, or
panel starts. DotMatch is scoped to assignment reliability, not downstream
screen statistics or genome alignment. Which workflow wrapper would make review
easiest for your lab: nf-core, MultiQC, Galaxy, Snakemake, or something else?

Release Follow-Up

The latest DotMatch release gates package installability, docs, scientific
claim boundaries, workflow examples, and public evidence checks before release
tagging. The project is looking for reviewed workflow integrations and scoped
pilot feedback.

7. Maintainer Issue Templates

nf-core / Workflow Module Opening

Title: Add DotMatch known-target assignment module

DotMatch assigns fixed read windows to known short DNA targets and writes TSV,
JSON, FASTQ, and HTML outputs. This module proposal is scoped to assignment
artifacts and explicit unique/ambiguous/unmatched/invalid outcomes.

Review asks:
- command shape and metadata
- output contract
- container pinning
- fixture coverage
- MultiQC compatibility

MultiQC Opening

Title: Parse DotMatch assignment QC outputs

DotMatch writes sample QC, summaries, top-unmatched rows, and panel-safety
outputs for known-target sequencing assignments. A MultiQC module should expose
assignment rate, ambiguity rate, unmatched rate, invalid windows, and panel
safety status without implying downstream biological pass/fail calls.

8. Pilot Scorecard

Use this privately during pilots. Publish only quote-approved summaries.

Dimension

Score

Notes

Install worked from released channel

0-2

PyPI, Bioconda, source, or container

Input mapping was understandable

0-2

Target table, sample table, read window

Assignment failures were clearer

0-2

Ambiguous, unmatched, invalid, unsafe correction

Outputs fit existing workflow

0-2

TSV, JSON, FASTQ, HTML, MultiQC, notebook

Citation and methods text was usable

0-2

Version, command, ambiguity policy

Public adoption record approved

0-2

Only with explicit approval and public URL

Interpretation:

  • 0-4: do not publicize; fix product or docs first.

  • 5-8: useful private feedback; consider a follow-up pilot.

  • 9-12: candidate for quote-approved adopter record.

9. Adoption KPI Dashboard Spec

Track exposure health separately from scientific evidence.

KPI

Source

Cadence

Homepage visits to install clicks

site analytics if enabled

monthly

Docs visits to tutorial starts

docs analytics if enabled

monthly

External workflow PRs opened

GitHub URLs

weekly during push

External workflow PRs merged

accepted public records

release cycle

Public pilot records approved

docs/adopters/

release cycle

Citation artifacts generated

release or assay outputs

release cycle

Distribution channel health

make distribution-channels

release cycle

Do not combine these KPIs with performance or correctness claims. Exposure can increase before external adoption is proven.

10. Release Communications Calendar

Use this around each release or major integration push.

Time

Action

Evidence link

T-7 days

Confirm claim boundary and release notes

docs/scientific-claims.md

T-5 days

Prepare maintainer issue or PR drafts

docs/workflow-submissions.md

T-3 days

Prepare short social and forum posts

this page

Tag day

Announce only after release workflow artifacts are visible

release URL

T+1 day

Verify PyPI, Bioconda, containers, Zenodo as applicable

docs/distribution-release.json

T+7 days

Follow up with maintainers and pilot contacts

public URLs only

T+30 days

Update adoption KPI snapshot

private tracker or public records

Completion Rule

These wins are complete only when the homepage, docs index, exposure kit, structured plan, and site guard all reference the same ten items. If the JSON plan and markdown playbook diverge, treat the playbook as not release-ready.