DotMatch

User Guide

  • Getting Started
  • Command Reference
  • CRISPR Count First Run
  • DotMatch to scverse for Perturb-seq and Feature Barcodes
  • Streaming Python API
  • DotMatch AssaySpec v1
  • DotMatch CRISPR Count QC
  • DotMatch Barcode Panel Design
  • DotMatch Workbench
  • DotMatch Proposals, Performance Ideas, and Bioinformatics Adoption Roadmap

Reference

  • DotMatch Public Schemas
  • Methods and Citation Template
  • Citation Flywheel
  • DotMatch Industry Exposure Kit
  • DotMatch Next 10 Industry Exposure Wins
  • Packaging Notes
  • Release Process
  • Workflow Submission Pack
  • Reviewer Evidence Matrix
  • Commercial Boundary
  • Evidence Packet v1

Evidence and Boundaries

  • Trust, Scope, and Evidence
  • DotMatch Evidence Notes
  • Barcode Validation Notes
  • Usability Comparison
  • Native Comparator Scope
  • Benchmark Report
  • DotMatch Evidence Gallery
    • Gallery
    • Report Examples
    • How To Read These Pages

Detailed Reports

  • Amplicon/Panel Assignment Evidence
  • Barcode Demultiplexing Benchmark
  • Barcode Panel Design Benchmark
  • BCL Demultiplexing Benchmark
  • CRISPR Comparison Evidence
  • Feature Barcode Assignment Evidence
  • Experimental GPU Acceleration Benchmark
  • Production CRISPR CPU vs Metal
  • Native Edlib Benchmark Report
  • Oligo/Adapter Assignment Evidence
  • Perturb-Seq And CRISPR Guide-Capture Assignment Evidence
  • Public CRISPR Workflow Comparator
  • Real CRISPR Benchmark
  • DotMatch Report Examples
  • Amplicon primer-start assignment: ARTIC V3 public lane
  • Barcode autopsy: weak signal and unsafe rescue
  • Public barcode demux: SRP009896 exact-prefix lane
  • Barcode failure fixture: unsafe one-edit correction
  • Barcode failure fixture: wrong offset and invalid windows
  • Classic BCL parser milestone: 10x tiny-BCL lane
  • Feature barcode assignment: 10x TotalSeq-B public lane
  • Oligo/adapter prefix assignment: TruSeq R1 public lane
  • CRISPR guide capture extraction: 10x GEM-X public lane
  • Public CRISPR guide counting: Yusa/MAGeCK lane
  • Barcode Troubleshooting Report
  • DotMatch Used By and Adopter Notes
DotMatch
  • DotMatch Evidence Gallery
  • View page source

DotMatch Evidence Gallery

A curated map of committed DotMatch benchmark reports, public-data examples, and diagnostic report patterns.

Start here when you want to see what DotMatch does on real or deliberately diagnostic data. Each row links to the exact report, raw artifacts, command records, and a short interpretation.

Gallery

Scenario

Category

Dataset

What it shows

Primary report

Public CRISPR guide counting: Yusa/MAGeCK lane

Public data

MAGeCK/Yusa public CRISPR guide-counting FASTQs

Known-good guide-counting lane with count agreement and Edlib validation.

report

Public barcode demux: SRP009896 exact-prefix lane

Public data

SRP009896/SRR391079 inline-barcode FASTQ subsample

Exact fixed-position barcode demultiplexing with documented Cutadapt and hash baselines.

report

Barcode autopsy: weak signal and unsafe rescue

Report zoo

SRP009896/SRR391079 barcode autopsy fixture

A review-status run with low-confidence offset evidence, low assignment rate, high ambiguity, and unsafe one-edit correction.

report

Barcode failure fixture: wrong offset and invalid windows

Report zoo

Committed barcode failure-mode FASTQ fixture

Deliberately constructed reads show wrong offset, invalid window, reverse-complement candidate, and low-complexity failure modes.

report

Barcode failure fixture: unsafe one-edit correction

Report zoo

Committed barcode failure-mode FASTQ fixture

Observed barcode sequences are within one edit of multiple targets, so k=1 rescue is unsafe.

report

Feature barcode assignment: 10x TotalSeq-B public lane

Public data

10x Genomics TotalSeq-B antibody Feature Barcode R2 sample

Per-read fixed-window feature-barcode assignment with exact-slice baseline agreement.

report

CRISPR guide capture extraction: 10x GEM-X public lane

Public data

10x GEM-X A375 CRISPR Guide Capture R2 sample

Single-guide fixed-window guide-capture extraction with exact-slice baseline agreement.

report

Amplicon primer-start assignment: ARTIC V3 public lane

Public data

nf-core viralrecon ARTIC V3 Illumina R1 sample

Fixed-window primer-start assignment with exact-prefix baseline agreement.

report

Oligo/adapter prefix assignment: TruSeq R1 public lane

Public data

fast-adapter-trimming TruSeq R1 fixed-window sample

Fixed-window adapter-prefix assignment with exact-slice baseline agreement.

report

Classic BCL parser milestone: 10x tiny-BCL lane

Gated parser milestone

10x Genomics Cell Ranger tiny-BCL demo run folder

Classic per-cycle BCL output count-total validation against an available bcl2fastq row.

report

Report Examples

Open the report examples for clean runs, weak barcode signal, wrong-window symptoms, unsafe correction, and validation milestones.

How To Read These Pages

  • Each scenario separates the observed result from what it does not establish.

  • Comparator settings are part of the evidence. Changing them changes the meaning of the result.

  • HTML, TSV, JSON, FASTQ, and raw CSV files are linked directly when they are committed.

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