Public barcode demux: SRP009896 exact-prefix lane
Category:
Public dataAssay type:
inline_barcodeEvidence level: Documented example
Dataset: SRP009896/SRR391079 inline-barcode FASTQ subsample
Condition shown: Exact fixed-position barcode demultiplexing with documented Cutadapt and hash baselines.
Primary report: docs/benchmarks/barcode_demux/README.md
What This Shows
DotMatch can reproduce a narrow exact-prefix inline-barcode demux comparison under documented semantics.
Speed numbers are attached to comparator settings rather than reported in isolation.
What It Does Not Show
This does not replace general adapter trimming.
This does not cover BCL/CBCL demultiplexing or arbitrary barcode placement.
Comparator And Validation
Comparator semantics: The checked public lane uses fixed-position k=0 DotMatch demux, Cutadapt anchored no-indel demux, and a transparent exact-prefix hash splitter baseline.
Validation: The barcode comparison gate requires evidence-ready metadata, five successful real-data repeats, assigned reads, Cutadapt rows, and the exact-prefix baseline row.
Report Examples
Barcode demux benchmark report (
markdown)
Raw Artifacts
Reproduce Or Check
make bench-barcode-comparisonmake barcode-comparison-gate