Barcode failure fixture: wrong offset and invalid windows

  • Category: Report zoo

  • Assay type: inline_barcode

  • Evidence level: Small fixture

  • Dataset: Committed barcode failure-mode FASTQ fixture

  • Condition shown: Deliberately constructed reads show wrong offset, invalid window, reverse-complement candidate, and low-complexity failure modes.

  • Primary report: examples/barcode_autopsy/failure_modes/README.md

What This Shows

  • DotMatch documents what wrong fixed-window coordinates and invalid extraction windows look like.

  • The fixture gives developers and users concrete examples of failure classifications.

What It Does Not Show

  • This fixture is synthetic and should not be used for performance claims.

  • Reverse-complement findings are advisory; users must normalize assay inputs themselves.

Comparator And Validation

Comparator semantics: The fixture is a deterministic diagnostic fixture for barcode failure classifications, not a public dataset comparator.

Validation: The barcode failure fixture checker requires the expected failure-mode table and matching fixture files.

Report Examples

Raw Artifacts

Reproduce Or Check

  • make barcode-validation-ready