# CRISPR guide capture extraction: 10x GEM-X public lane - Category: `Public data` - Assay type: `crispr` - Evidence level: Validation milestone - Dataset: 10x GEM-X A375 CRISPR Guide Capture R2 sample - Condition shown: Single-guide fixed-window guide-capture extraction with exact-slice baseline agreement. - Primary report: [docs/benchmarks/perturb_seq/README.md](../../benchmarks/perturb_seq/README.md) ## What This Shows - DotMatch can extract and count a fixed-window guide-capture signal against one observed guide target. - The paired diagnostic fixture still checks side-level ambiguity handling. ## What It Does Not Show - This is not useful multi-guide assignment evidence. - This is not guide-per-cell calling. - Expression processing and perturbation-effect analysis remain outside DotMatch. ## Comparator And Validation Comparator semantics: The checked public lane uses a 10x CRISPR Guide Capture R2 subsample, one observed guide target, an observed fixed guide window, and a transparent exact-slice hash baseline. Validation: The public gate requires synthetic pair-count diagnostics, public DotMatch k=0 and k=1 rows, zero validation mismatches, exact agreement with the k=0 baseline, and k=1 assignment count no lower than k=0. ## Report Examples - [Perturb-seq/guide-capture benchmark report](../../benchmarks/perturb_seq/README.md) (`markdown`) ## Raw Artifacts - [benchmarks/raw/perturb_seq.csv](../../../benchmarks/raw/perturb_seq.csv) - [examples/perturb_seq/data/metadata.json](../../../examples/perturb_seq/data/metadata.json) ## Reproduce Or Check - `make bench-perturb-seq-public` - `make perturb-seq-public-gate`